Ecocyte Bioscience MTS - Modified Tyrodes Solution (1000 ml) - 20x concentrate

Description

Modified Tyrode's Solution for use with mammalian heart tissue. Product information 20x concentrate MTS solution Carbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering. autoclaved Instructions for use: 20x concentrate: For 1000 ml MTS Solution: Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water Add slowly 50 ml Base B (20x) Oxygenate ACSF Solution during use pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene) Use immediately once opened. Store at 4 - 8 °C Ional composition of MTS Solution (Base A + Base B) NaCl = 126,7 mM KCl = 5,4 mM CaCl2 = 1,8 mM MgCl2 = 1,05 mM NaH2PO4 = 0,42 mM NaHCO3 = 22 mM Glucose = 5 mM