Western blot is a widely used method for protein analysis in bio-medical research. The most difficult and annoying factor in getting good result is non-specific binding of antibody to the membrane, which causes high background/noise in Western blot. It is even more difficult to detect phospho-protein in Western blot because of its specific nature. This blocking buffer from KingXo is able to significantly increases ratio of signal and noise. it can save technicians and scientists a lot of time and money when use it in the lab. It is particularly suitable for decreasing the high background of "bad", "dirty" or "not working well" phospho-specific antibodies with a wide range of affinity in Western blot.
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